| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.071923 |
| Chromosome: | chromosome 1 |
| Location: | 7897227 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g055453 | ALS2,ALSS1 | Acetolactate synthase, small subunit; (1 of 1) K01653 - acetolactate synthase I/III small subunit (E2.2.1.6S, ilvH, ilvN) | 3'UTR |
| Cre01.g055457 | (1 of 15) PTHR12291//PTHR12291:SF23 - FAMILY NOT NAMED // PROTEIN MEC-15 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATTAAGCTCACACAGGAGTATCTGTGCCGCCTGAAGGCTGCTGTAAACA |
| Internal bar code: | GTGAGCGCATTGTCAGTTGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 865 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGACACGGCTTCAGGAG |
| Suggested primer 2: | CAGCCTACCTGCGTATGGAG |