| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071935 |
| Chromosome: | chromosome 13 |
| Location: | 4646611 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603900 | TSF1 | (1 of 1) K01890 - phenylalanyl-tRNA synthetase beta chain (FARSB, pheT); Phenylalanyl-tRNA synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACCAATTGAGTGTGAGGTGGGGCGGGGTGAAGGCGCGTGCGTGCATGGC |
| Internal bar code: | GGGGTGCTTGGAGCCAAACAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1569 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCTAGCCATCCCCATCACA |
| Suggested primer 2: | AATCATGACACGGCGACTGA |