| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071950 |
| Chromosome: | chromosome 2 |
| Location: | 8282660 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g143350 | PDE6 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACAGCGGTCCGTTGGAGGGCAAGGCCTCTGCTGTTGCCTTGCACTGA |
| Internal bar code: | CATTTTGAGACCGCTAGTCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1871 |
| LEAP-Seq percent confirming: | 38.4615 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGCTGCATTCATACGC |
| Suggested primer 2: | GTCAAGGGTGAGTGTCTGGG |