| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.071957 |
| Chromosome: | chromosome 12 |
| Location: | 1841130 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512350 | 3'UTR | ||
| Cre12.g512400 | (1 of 1) PTHR30224 - ELECTRON TRANSPORT PROTEIN | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACCTCATCTGGTGCAACTAGCTGGTCAAAGAACCGTACAGTCCGGGTG |
| Internal bar code: | CTTAATTCTTATTCACGTACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2066 |
| LEAP-Seq percent confirming: | 93.9394 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCATCTCATTGCTGACTGCG |
| Suggested primer 2: | GAGATGGACGGCCTCATCTG |