Insertion junction: CLIP2.071964_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.071964
Chromosome:chromosome 1
Location:5055861
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g035150 (1 of 19) PF00642 - Zinc finger C-x8-C-x5-C-x3-H type (and similar) (zf-CCCH) 5'UTR
Cre01.g035200 (1 of 13) 2.3.1.225 - Protein S-acyltransferase / S-protein acyltransferase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGTAGCTATCTTGTCAGCAATACACTTTGATTAGCTAATGTGTCGCAAG
Internal bar code:TAGACGGAATACATATAACGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1395
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GCACATGCAACAGCAAGGAA
Suggested primer 2:TCTCTGACCGTGATTGCACC