| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071967 |
| Chromosome: | chromosome 3 |
| Location: | 2421857 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158700 | (1 of 1) PTHR10157:SF23 - DBH-LIKE MONOOXYGENASE PROTEIN 2-RELATED | 3'UTR | |
| Cre03.g158750 | HEL14 | (1 of 1) PTHR24031:SF306 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 11; DEAD/DEAH box helicase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGAATGTACAACAGCTGCAGCAACAACACACCGTGGATGTGGCCGGG |
| Internal bar code: | CAGCACGGCGATACAGTCTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5194 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAGCCGTCATTCTCACA |
| Suggested primer 2: | TGCCATTGTCGTCGGTTGTA |