| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.071997 |
| Chromosome: | chromosome 9 |
| Location: | 3952622 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g398326 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATACTGAAATACAATTAGTTTGTGCGGCGTTGGGGTCTGGAGGCGGCGC |
| Internal bar code: | CGTAATACTCACGGGGAAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2229 |
| LEAP-Seq percent confirming: | 44.4444 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAACGTGGCTGTACAGG |
| Suggested primer 2: | AGTAAGTGAGCCGCGTCAAA |