| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.071998 |
| Chromosome: | chromosome 16 |
| Location: | 6251745 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g676400 | HEL63 | (1 of 1) K12811 - ATP-dependent RNA helicase DDX46/PRP5 (DDX46, PRP5); DEAD/DEAH box helicase | 5'UTR |
| Cre16.g676402 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCGCCATTTGAGTCACACGCGATGAAGTAGATGCAGATTTTTCCTTAA |
| Internal bar code: | TTCGCCCTGGACGGGTTCACTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1835 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTCACGGTCGTGGTCTTTC |
| Suggested primer 2: | TGCAGAGCCAACACGTACAT |