Insertion junction: CLIP2.072001_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072001
Chromosome:chromosome 14
Location:1104840
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g614750 CPNAT1,cpNAT1,NAT34 (1 of 1) 2.3.1.189 - Mycothiol synthase; Ribosome-associated chloroplast-localized N-acetyltransferase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGAATGCATTGGTTACAAGAGGGACTTGTGGACACGAGTCGCAAGCGAT
Internal bar code:TATCTACTCAGCGATGGGGAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1094
LEAP-Seq percent confirming:58.9286
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:23
LEAP-Seq n unique pos:56

Suggested primers for confirmation by PCR

Suggested primer 1:CTTGGTTTCGCTGCGTTTCA
Suggested primer 2:CCCTCCCCATCTTTGCCAAT