| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072008 |
| Chromosome: | chromosome 7 |
| Location: | 5539117 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g351600 | HEL40 | (1 of 2) PTHR24031:SF303 - DEAD-BOX ATP-DEPENDENT RNA HELICASE 47, MITOCHONDRIAL; possible helicase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCACTTCAGTATTTTCCTGCGACGAAACTACTACTGCAATTATAAGCA |
| Internal bar code: | AGGTAAAAGTGTGGAGAGACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3404 |
| LEAP-Seq percent confirming: | 95.8333 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGATGAACGGCTTAACGCG |
| Suggested primer 2: | ATACTGTAGGGCGGGGTTCT |