Insertion junction: CLIP2.072015_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.072015
Chromosome:chromosome 3
Location:6312248
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g192201 PHC51 Pherophorin-chlamydomonas homolog 51; (1 of 71) PF12499 - Pherophorin (DUF3707) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTGATCGAACAACACACGGCGCCAATCGGGCTGCAATGGTGCTGTCTCC
Internal bar code:ACCTCGTGACGAGTCCCGTAGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2821
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:81

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCGTGAACACATTGTGCA
Suggested primer 2:GCCACTCTGTGCTTCTCACT