| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072017 |
| Chromosome: | chromosome 13 |
| Location: | 4578936 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603450 | (1 of 4) PF14159 - CAAD domains of cyanobacterial aminoacyl-tRNA synthetase (CAAD) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTGCGAGCGGTCCCGCGCGCACTCCGGCGCGAGCTCGCGCCGAACTGTG |
| Internal bar code: | CTACTCGGTACTTAGTATGTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1765 |
| LEAP-Seq percent confirming: | 76.9231 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 39 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTTGGGGTAGCTTGGAC |
| Suggested primer 2: | TGTGTGTTGGGAGGCTTTGT |