| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.072021 |
| Chromosome: | chromosome 9 |
| Location: | 5696377 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g410350 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCGACTTCCCAGACGTCACAGGAGGCAGCGACCGGGCCCGGCGGCGGC |
| Internal bar code: | TGTTGTTCGTAAAACCCGTGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 449 |
| LEAP-Seq percent confirming: | 3.57143 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 27 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCTCGCCACATTCTCCA |
| Suggested primer 2: | ACAGCCGATTGCAATCTCCA |