| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072028 |
| Chromosome: | chromosome 3 |
| Location: | 2761988 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g161500 | (1 of 1) PTHR23041:SF65 - E3 UBIQUITIN-PROTEIN LIGASE CCNB1IP1 HOMOLOG | 3'UTR | |
| Cre03.g161550 | FAP55 | (1 of 1) IPR009053//IPR023220 - Prefoldin // Type IV secretion system, VirB5-domain; Flagellar Associated Protein 55 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTATACCTGCAGGCAAGAGGCCTGATTCATGTAGCCCAAGCTGACCGCT |
| Internal bar code: | GTTTATGGATGACGAGTTCAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5180 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 74 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGTGCACCCAAGCTGTG |
| Suggested primer 2: | TTGACCAGCACGTGCATACT |