| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072030 |
| Chromosome: | chromosome 12 |
| Location: | 2770954 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504050 | LAG2 | Predicted protein related to longevity assurance protein; (1 of 5) 2.3.1.24 - Sphingosine N-acyltransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGAAAGACTGAAAGTTCGGGCATGCATGACCCCCGCTAGGTCCCGATCA |
| Internal bar code: | TGTAAACTACGCACATGCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1621 |
| LEAP-Seq percent confirming: | 33.9623 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACTTTTCGGCGCAAGGT |
| Suggested primer 2: | ATCACTGGGTGGTCAGCTTG |