| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072033 |
| Chromosome: | chromosome 12 |
| Location: | 2592883 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g506550 | 5'UTR | ||
| Cre12.g506600 | LPN1,PAH1 | Putative phosphatidate phosphatase; (1 of 1) K15728 - phosphatidate phosphatase LPIN (LPIN) | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACCTCTCCAGCTCATGTCCTTGACACGGTCGGTGCACATGCATGTCT |
| Internal bar code: | ATACCCGATTGGTGTGTCCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2275 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATGAGAGGAGGCTAGGT |
| Suggested primer 2: | CGCTGATGTTGCCTTCATCG |