Insertion junction: CLIP2.072033_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.072033
Chromosome:chromosome 15
Location:2191576
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre15.g801755 (1 of 2) PF00665//PF07727//PF13976 - Integrase core domain (rve) // Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_2) // GAG-pre-integrase domain (gag_pre-integrs) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGCTACTACCGGCGACCTCCTGCGTTGCAGCACTGCTGTCGGTTTCG
Internal bar code:GTTTGATCCTATACAGTGTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:143
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGCAAGGTGGTGTTTGAG
Suggested primer 2:GGCGACAGGTTGCGGATATA