| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072044 |
| Chromosome: | chromosome 17 |
| Location: | 1638581 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g707650 | SNRK2L | (1 of 4) PTHR24343//PTHR24343:SF167 - SERINE/THREONINE KINASE // SUBFAMILY NOT NAMED; snRK1 family in Chlamydomonas, subgroup 2 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGAGAACTCCCGTGCTAGCAGTCGCAGATGATACTCTACGTGCGTATAA |
| Internal bar code: | GGCGGTCTGCAAGATGGGCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3389 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 70 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGTAGGCAACCAAGGACA |
| Suggested primer 2: | GTCCCCACTTGAAGGGTCAG |