| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072048 |
| Chromosome: | chromosome 3 |
| Location: | 5614022 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185850 | CPK2 | (1 of 1) PTHR10584//PTHR10584:SF162 - SUGAR KINASE // SUBFAMILY NOT NAMED; putative carbohydrate kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAGAGTGATGGAGATGGAGTTCGGGTACGAGAGGGCTTGAATTCAAACG |
| Internal bar code: | AGTCTGAGATTGCATAGTGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 2.77778 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAACACACTGGGACCTC |
| Suggested primer 2: | AGGCTGCTTAGGGTTTGGAC |