Insertion junction: CLIP2.072063_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072063
Chromosome:chromosome 17
Location:3513843
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g724400 FAP257 Flagellar Associated Protein 257; (1 of 3) PF05217 - STOP protein (STOP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGGATCCTACCGGCACGCGTATCATTGTCCACCACACCTGCATCACG
Internal bar code:AGGTCGAATTTTGTCGTGCAAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2529
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:92

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATACTAGCCCTGACCCT
Suggested primer 2:GAGAAGTGCGACAGGGGAAA