| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072071 |
| Chromosome: | chromosome 1 |
| Location: | 3641163 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g023650 | Probable amino acid/metabolite permease; (1 of 4) PTHR11785:SF367 - AMINO-ACID PERMEASE BAT1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTCATGAATGTGTGTGGATGTGAGAGTGAGGGTCTCGCACGGGCGAC |
| Internal bar code: | TATTGCATGAGGGGAAGGACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 155 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTGCACACCTCCTTCTG |
| Suggested primer 2: | CCGCTCTGTGTTTTGACACG |