Insertion junction: CLIP2.072072_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.072072
Chromosome:chromosome 12
Location:1932903
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g511700 (1 of 1) K14066 - geranyl diphosphate synthase (GPS) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCTTTCCAGTTCGGTGTTTACAACGGCTAGGGCCGGGGTCGACACTACG
Internal bar code:AAGCAAGAAGAGCTTTGAACAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1730
LEAP-Seq percent confirming:94.0
LEAP-Seq n confirming:47
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:50

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAGGCAATCGAGGAATTG
Suggested primer 2:GACAACACGCAACAAGGGAC