Insertion junction: CLIP2.072078_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072078
Chromosome:chromosome 9
Location:4188991
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre09.g399738 (1 of 239) IPR016024 - Armadillo-type fold CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTTGAGTGGCAACGGCATGGGCGCAGGCAGCATTAACACGGGCAAGCTG
Internal bar code:AGATCAGGGTCATGGTCCCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:470
LEAP-Seq percent confirming:34.7826
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:TACTGGCGGTCTTTGGACAC
Suggested primer 2:AACGAACTTTGGTCGCTTGC