Insertion junction: CLIP2.072083_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.072083
Chromosome:chromosome 2
Location:1873658
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre02.g087450 (1 of 4) PTHR16255:SF4 - SPORULATION PROTEIN RMD8 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACACACACACACACACACACACACACACACACACACACACACACACA
Internal bar code:CCTTCTTAACGCAAAACTCCAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1777
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GGGTGCATGGACTGTGTGTA
Suggested primer 2:CCTCTCCCTTCCACACACAC