| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072085 |
| Chromosome: | chromosome 3 |
| Location: | 6057395 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g190100 | EIF3B | (1 of 1) K03253 - translation initiation factor 3 subunit B (EIF3B); Eukaryotic translation initiation factor 3, subunit B | 5'UTR |
| Cre03.g800353 | (1 of 1) K06631 - polo-like kinase 1 (PLK1) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGCTTTTGTGCGTGAATCTAGCAATTCCTTTTTCTGTGAGCCATACAGC |
| Internal bar code: | GAGCTAGGTTGTATGTTATTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4235 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 72 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGTATCCACAGCGAGGCTC |
| Suggested primer 2: | GATCCGCACGTTTCACATCG |