| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072086 |
| Chromosome: | chromosome 12 |
| Location: | 1696034 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486250 | ARL6,ARFA1A,FAP333 | (1 of 2) PTHR11711:SF181 - ADP-RIBOSYLATION FACTOR A1F; Flagellar Associated Protein 333 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAACGCAATCCAAACTCCTACCACCAAGGCTGTGAATGGCTTCCTTTT |
| Internal bar code: | CTCGCGCGGAGGTGGTTTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1739 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCAGGGCCCGCATGTAA |
| Suggested primer 2: | AAGCTCTTTCCCCATCCAGC |