| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072091 |
| Chromosome: | plastome |
| Location: | 76993 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802295 | ChreCp030,psbN,2717031 | photosystem II protein N; (1 of 1) K02715 - PsbN protein (psbN) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATAACTAAAGTTTGTTACTTTACCGAAGACGTTTACCCTTTCTCGGTTAA |
| Internal bar code: | TTCGGACTTCCTCCTTTAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 90.9091 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGGCAGTCCCTTTTCCCC |
| Suggested primer 2: | GAAGGGGAAGGACGTCAGTG |