Insertion junction: CLIP2.072094_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.072094
Chromosome:chromosome 10
Location:627375
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g421900 SRR27 Scavenger receptor cysteine rich (SRCR) protein; (1 of 5) 1.4.3.13 - Protein-lysine 6-oxidase / Lysyl oxidase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTGAACGTACTCATTCCGCCCTCCGATCTGGCTCGTGTGTGCGTGTGTG
Internal bar code:GCAAGAGCTAGGAAAACCATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1058
LEAP-Seq percent confirming:7.31707
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:38
LEAP-Seq n unique pos:41

Suggested primers for confirmation by PCR

Suggested primer 1:AAGACATGACTTGCCTGCGA
Suggested primer 2:TGCTACATCATCGACGACGG