Insertion junction: CLIP2.072104_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.072104
Chromosome:chromosome 10
Location:5732270
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g459600 (1 of 1) K14832 - ribosome biogenesis protein MAK21 (MAK21, NOC1, CEBPZ) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCGCCCCGCGCCGCTCCAGCACCGCCGCCCGCACTGTCCTAGTCCGT
Internal bar code:GTCATGTGGGGGCGAGTCATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:652
LEAP-Seq percent confirming:90.9091
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GACTACGAGGCGCTGATTGA
Suggested primer 2:AGCTCCCCAAGTAAACCACG