| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.072106 |
| Chromosome: | chromosome 3 |
| Location: | 803150 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g146247 | NUOS4 | NADH:ubiquinone oxidoreductase 18 kDa subunit; (1 of 2) K03937 - NADH dehydrogenase (ubiquinone) Fe-S protein 4 (NDUFS4) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCTTTAGGGGCTCGCCTGCACTCAAGAGTCAACTAGTTACAAATGCAGC |
| Internal bar code: | ATCATAAGTTAAGATCTTGCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGCGCATGTTTGGACAACA |
| Suggested primer 2: | ATGTACGAGGAGATGCAGCG |