| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072137 |
| Chromosome: | chromosome 4 |
| Location: | 1837421 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g217900 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGTGGACCGACCTCCGCGCAACGGGCCGCAAGTCAGCGGAGCGAGT |
| Internal bar code: | CATTACGTTTATTTAGCATCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1418 |
| LEAP-Seq percent confirming: | 77.5 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTAGGGATGCGCCAGAGAAG |
| Suggested primer 2: | GAAGGCGTAGATGGGGATGG |