| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072141 |
| Chromosome: | chromosome 14 |
| Location: | 3056801 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g628000 | TMX1 | tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase; (1 of 1) 2.1.1.61//2.8.1.4//2.8.1.7 - tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase // tRNA sulfurtransferase // Cysteine desulfurase / Cysteine desulfurylase | 5'UTR |
| Cre14.g628050 | COG1 | Component of oligomeric golgi complex; (1 of 3) PF08700 - Vps51/Vps67 (Vps51) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCACGTCACGTAAATGTATGTATGCGGCAAGCTTGCTATAGTGTACCA |
| Internal bar code: | TTAACGCCATCTACAACAATAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1647 |
| LEAP-Seq percent confirming: | 98.0 |
| LEAP-Seq n confirming: | 49 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCAGCATTGTGCCATCAAA |
| Suggested primer 2: | GTCTGAGGGGGTTAGGGCTA |