| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072145 |
| Chromosome: | chromosome 6 |
| Location: | 2703899 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g270750 | (1 of 3) PF07887 - Calmodulin binding protein-like (Calmodulin_bind) | 5'UTR | |
| Cre06.g270800 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAATAGACTCTGATGTATTGTTGACAATGCAAGCAGAGACTGTGGCTGT |
| Internal bar code: | TAAGGCAAATCAAGTTGAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1492 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCTCCTTCTTCTTGCCCTT |
| Suggested primer 2: | GCCAGCAAGCAACAAACTCA |