| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072159 |
| Chromosome: | chromosome 7 |
| Location: | 3446260 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g335550 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCGCAGATGGCTACCCCTACTGCGTTACTCGGCGCGGCCGGCCCCGG |
| Internal bar code: | CCCTTAGTACAGTCGCGGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4081 |
| LEAP-Seq percent confirming: | 89.8305 |
| LEAP-Seq n confirming: | 53 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCGGAGATGGATGAGAAGG |
| Suggested primer 2: | CACACCCGTCGAACAAAACC |