| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072162 |
| Chromosome: | chromosome 7 |
| Location: | 3090390 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g800801 | (1 of 352) PF00078 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCTGCAACATTTTTTTTTTTTGAGGAATCATCCTTACAAGGTTGAACA |
| Internal bar code: | GGGTATTGGTGACGTTATCTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 192 |
| LEAP-Seq percent confirming: | 7.14286 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGCGCCCATGAGTACTAG |
| Suggested primer 2: | ACAATAACCCGGTGGCTCTG |