| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072173 |
| Chromosome: | chromosome 1 |
| Location: | 5295750 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g037200 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCCTGCCACCCATACACAAAAGCAATCAATTTGCACATCTAACGCCT |
| Internal bar code: | TTCTCTTGTGGCGAGAGTCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1989 |
| LEAP-Seq percent confirming: | 76.0 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTATAGCCGTCGTGAGCAT |
| Suggested primer 2: | ACGCCCTAGGTGACCTTTTG |