Insertion junction: CLIP2.072177_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.072177
Chromosome:chromosome 5
Location:3067360
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre24.g755447 (1 of 1) K12863 - protein CWC15 (CWC15) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGCTGGGTCCGCCAGGTCCCCCCTCCATCCATGTTGGGTAGGTATTGTA
Internal bar code:TAGGGCATGCGAGTGTTCAGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:199
LEAP-Seq percent confirming:26.6667
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:AGCTTCTTCAATCCGCACCA
Suggested primer 2:CCGATCCTCCACAATCTCCG