| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.072178 |
| Chromosome: | chromosome 11 |
| Location: | 932502 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467657 | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGCACGAACACCTGGACGCGACTGGACGCACGTTGGGAATGCAGCATG |
| Internal bar code: | TTCTTCCCTGCTATCCTATTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1119 |
| LEAP-Seq percent confirming: | 94.4444 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 36 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTACTGAGCCGCAACACAG |
| Suggested primer 2: | GCCCTCCGTCTCAGACAAAA |