| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.072179 |
| Chromosome: | chromosome 5 |
| Location: | 3473697 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233450 | ELG30 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 30 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGACTCTGTAAGGGGCTTGACCTTCGTTACCCGGCTCGGACGTGCGTGA |
| Internal bar code: | GGTTTGCTTTCTCTTAATCGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1415 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGGGGAAAGGGGATGTGG |
| Suggested primer 2: | GGGGCGAGTCGTATACGTTT |