| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072184 |
| Chromosome: | chromosome 9 |
| Location: | 5820719 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g411450 | DAP1,DNAAF2,Ktu,MOT45,PF13,PRR1 | (1 of 3) PF08190 - pre-RNA processing PIH1/Nop17 (PIH1); Dynein Assembly factor PIH domain 1 | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAACAAAGACTTGCTCATTAGTTTCACAACTACAAGGAAGCTCTGTTGCG |
| Internal bar code: | TGCAGGAGTCATCGTTTAGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 45.977 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 47 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCATCTCCTGCAGCAGTT |
| Suggested primer 2: | TGCTACGCCCAATAACAGCA |