| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.072187 |
| Chromosome: | chromosome 2 |
| Location: | 7264015 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391282 | XYT1 | (1 of 1) K03714 - glycoprotein 2-beta-D-xylosyltransferase (E2.4.2.38); Xylosyl transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCAGCGAACGCGATGGGGCGGATGCATTCCCGCCCTTTAGGGCGCCAGT |
| Internal bar code: | TTGTTTGGTTTAATGAATTCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1149 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 22 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTCACGACAACGGTCTT |
| Suggested primer 2: | CACTTAGGCATGCACATCGC |