| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072194 |
| Chromosome: | chromosome 16 |
| Location: | 847366 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g647700 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACTTGGTAGTGGCGAAGCCACTAATGACTAGGGCTGACAAGCCCTAGTG |
| Internal bar code: | ATCAATAGCGTATTTAAGAACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3500 |
| LEAP-Seq percent confirming: | 44.5652 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 51 |
| LEAP-Seq n unique pos: | 92 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGGGTGGGCTAGAGAGG |
| Suggested primer 2: | CAAGGCGTTCTAGCTGGTCA |