| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.072197 |
| Chromosome: | chromosome 3 |
| Location: | 352890 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144627 | CGS1 | Cystathionine gamma-synthase; (1 of 1) 2.5.1.48 - Cystathionine gamma-synthase / O-succinylhomoserine synthetase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGACGCTGGAGCTGCGCGTCACGCGCCACAACGCCAGCGCAATGGAGATC |
| Internal bar code: | TCGCTCGCTAAAGTTGAATTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 484 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGCTTACGGTTAGCTGCG |
| Suggested primer 2: | ATGAGTATGGGCGCTATGGC |