Insertion junction: CLIP2.072197_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.072197
Chromosome:chromosome 9
Location:944225
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g402900 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACCACCACCACCACCACACACGCATGTGCTGCACGCACCACATCGTCA
Internal bar code:ACAAGTAATCGGATTTAATGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:2329
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GTATCGCGACACACATGCAC
Suggested primer 2:CTTTTACTGAATCGCCGCCG