| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072201 |
| Chromosome: | chromosome 2 |
| Location: | 1191777 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g081400 | BCA1 | Branched chain amino acid aminotransferase; (1 of 1) 2.6.1.88 - Methionine transaminase / Methionine-oxo-acid transaminase | 5'UTR |
| Cre02.g081450 | CGL123,MRPL25,bL25m | (1 of 1) PTHR33284//PTHR33284:SF1 - FAMILY NOT NAMED // RIBOSOMAL L25/TL5/CTC N-TERMINAL 5S RRNA BINDING DOMAIN-CONTAINING PROTEIN; Mitochondrial ribosomal protein L25 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGCTGCGTTGGTTGGAAATGCTGACGCGGTGACTCTGGAGCTGACTGTG |
| Internal bar code: | GGTGTCGAGGCGGGGCTGTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1854 |
| LEAP-Seq percent confirming: | 82.9545 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 88 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGATGGAGGGGCTCATTCCT |
| Suggested primer 2: | ACCAAGACATGCTGGTACGG |