Insertion junction: CLIP2.072205_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.072205
Chromosome:chromosome 14
Location:580248
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AATCATCCTCAAACGCTGTCTGTGACCGACGCCAACTTACCAGATGTGCG
Internal bar code:CGATCGCTGGACCTCAGAGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2138
LEAP-Seq percent confirming:87.7778
LEAP-Seq n confirming:79
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:90

Suggested primers for confirmation by PCR

Suggested primer 1:GACAAACGGTTTCGCAGCTT
Suggested primer 2:CCATTGCTGCTGTTGCTGTT