| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.072208 |
| Chromosome: | chromosome 6 |
| Location: | 8775125 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311000 | FBT2 | (1 of 4) PTHR31585:SF0 - FOLATE-BIOPTERIN TRANSPORTER 1, CHLOROPLASTIC; putative folate/pteridine transporter 2 | outside_mRNA |
| Cre06.g311050 | (1 of 1) K00167 - 2-oxoisovalerate dehydrogenase E1 component beta subunit (BCKDHB, bkdA2) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGAGCTGATGGAACCGGTACCTCTCTGGACCCGGTCTCAAGATTTCAG |
| Internal bar code: | ATACCGGAAAGGTACAGGCGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 750 |
| LEAP-Seq percent confirming: | 20.7792 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGAAGTCACTTGAGCCGGT |
| Suggested primer 2: | GTTGCTAAGGCGCTGTATGC |