Insertion junction: CLIP2.072214_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.072214
Chromosome:plastome
Location:188123
Confidence (%):40
Locus systematic id Locus common name Defline Feature
CreCp.g802331 2716963,ChreCp066,psbC (1 of 1) K02705 - photosystem II CP43 chlorophyll apoprotein (psbC); photosystem II 43 kDa protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTTCATGGTTAGCTTGTTCACACTTCTGTTTAGGTTTCTTCTTCTTCAT
Internal bar code:GGAGTATGATGTTTGCGAAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:291
LEAP-Seq percent confirming:12.7273
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:48
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAGTCACAATCCACTGCC
Suggested primer 2:CGTGCTTTCGTTTGGTCTGG