| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.072222 |
| Chromosome: | chromosome 3 |
| Location: | 2337190 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158150 | (1 of 1) K12842 - U2-associated protein SR140 (SR140) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGCAGGCAACAGGATACGGTACGAGTGGAAACAGGAGGGGCAGGTGCA |
| Internal bar code: | GCACCGAGCGCTCGGGCGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 439 |
| LEAP-Seq percent confirming: | 3.1746 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 63 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCCCACTTAACCACCTCTCC |
| Suggested primer 2: | GGTGCTCCTGATGATACCGG |