| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.072245 |
| Chromosome: | chromosome 4 |
| Location: | 22332 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g213761 | (1 of 1) PTHR31793//PTHR31793:SF5 - FAMILY NOT NAMED // THIOESTERASE-LIKE PROTEIN | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAGATCCCGTGTGCATTGGCCAACACAATAAAAAATCAGTGTTTGATT |
| Internal bar code: | TGGTCGTTCGACGGTTTGTTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 863 |
| LEAP-Seq percent confirming: | 37.1429 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTATAGCAGTGGCGTGCACA |
| Suggested primer 2: | CTAAATGTCGCGTTCGCCAG |